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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and therefore may perhaps lead to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial role in endorsing mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are actually described. Two additional splice variants ended up noted but in depth nucleotide sequence information will not be but available.
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Blocking peptides are peptides that bind specially for the target antibody and block antibody binding. These peptide normally contains the epitope acknowledged from the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This manual outlines even more doable optimization of mobile and immunoassay parameters to make sure the very best success are...
Antibodies sure to the blocking peptide now not bind into the epitope on the concentrate on protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, you can see which staining is certain.
Concentrate on Facts Cyclin E belongs on the hugely conserved cyclin household, whose associates are characterized by a remarkable periodicity in protein abundance through the cell cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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